静磁场协同微生物降解烟煤孔隙结构演化及甲烷促解机制

Evolution of pore structure in bituminous coal and mechanism of methane promotion during microbial degradation under the synergy of static magnetic field

  • 摘要: 为探究静磁场协同微生物作用下烟煤煤体孔隙结构演化特征及甲烷促解机制,研究在常规厌氧培养体系基础上增设高低强度磁场处理组(0、20、40 mT),系统开展实验室甲烷析出速率试验、16S rRNA高通量测序、傅里叶变换红外光谱(FTIR)、扫描电镜试验(SEM)和低温液氮吸附−脱附试验(BET)。结果表明:20 d培养周期中,低磁场厌氧培养组(20 mT)的析出甲烷速率最高,为4.61 μmol/(g·h),较普通培养组提高了2.73倍;属水平群落分析显示,低磁场处理显著富集Clostridium_sensu_stricto_1菌种,丰度较对照组提升15%,乙酸营养性产甲烷古菌Methanosarecina占比达76%;功能基因注释指出,低磁场组脂肪酸代谢、甲烷代谢和苯甲酸代谢通路表达显著增强;红外光谱数据显示,静磁场的加入增加煤中脂肪类官能团—CH、—CH2、—CH3的占比,提高微生物作用位点数量,从而提高煤中原位微生物分解每种有机质速率;煤液氮吸附数据表明,低磁场组的氮气吸附量为原煤的2.25倍、普通培养组的1.48倍,微孔孔容占比达到76.68%,分形维数亦明显增大,孔隙结构趋于复杂;辅助SEM电镜观察煤样表面显示,在低磁场组表面可见更明显的煤体褶皱,且在同倍数下可见细菌附着,相比于其他组煤样,煤样裂隙发育更明显。研究结果揭示了煤样在不同静磁场强度影响下微生物菌群演变和孔隙结构的变化规律,以期为煤层瓦斯高效开采提供理论依据。

     

    Abstract: To explore the evolution characteristics of the pore structure of bituminous coal and the mechanism of methane promotion under the combined action of static magnetic field and microorganisms, this research, based on the conventional anaerobic culture system, added high and low intensity magnetic field treatment groups (0, 20, 40 mT), and systematically carried out laboratory methane production rate experiments, 16S rRNA high-throughput sequencing, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and low-temperature liquid nitrogen adsorption-desorption experiments (BET). The results show that: During the 20-day culture period, the methane production rate of the low magnetic field anaerobic culture group (20 mT) was the highest, reaching 4.61 μmol/(g·h), which was 2.73 times higher than that of the control group. At the genus level, the low magnetic field treatment significantly enriched the Clostridium-sensu-stricto-1 species, with an abundance increase of 15% compared to the control group, and the proportion of acetate-nutrient methane-producing archaea Methanosarecina reached 76%. Functional gene annotation indicated that the expression of fatty acid metabolism, methane metabolism and benzoic acid metabolism pathways in the low magnetic field group was significantly enhanced. Infrared spectroscopy data showed that the addition of static magnetic field increased the proportion of fatty acid functional groups —CH, —CH2, —CH3 in coal, increased the microbial action sites, and thus increased the rate of in-situ microbial decomposition of each organic matter in coal. The liquid nitrogen adsorption data of coal indicated that the nitrogen adsorption capacity of the low magnetic field group was 2.25 times and 1.48 times that of raw coal and the control group, respectively, and the proportion of micropore volume reached 76.68%, and the fractal dimension also significantly increased, indicating a more complex pore structure. The SEM observations of the coal samples’ surfaces in the low magnetic field group showed more obvious coal body folds, and bacteria were visible at the same magnification. Compared with other groups of coal samples, the coal sample fissures were more developed. The research results reveal the evolution patterns of microbial communities and changes in pore structure under the influence of different static magnetic field intensities in coal samples, aiming to provide a theoretical basis for the efficient extraction of coalbed methane.

     

/

返回文章
返回